• Aucun résultat trouvé

Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips

N/A
N/A
Protected

Academic year: 2021

Partager "Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips"

Copied!
19
0
0

Texte intégral

(1)

Any correspondence concerning this service should be sent

to the repository administrator:

tech-oatao@listes-diff.inp-toulouse.fr

This is an author’s version published in:

http://oatao.univ-toulouse.fr/27310

To cite this version: Gavira, Jose A. and Rodriguez-Ruiz,

Isaac and Martinez-Rodriguez, Sergio and Basu, Shibom and

Teychené, Sébastienand McCarthy, Andrew A. and

Mueller-Dieckman, Christoph Attaining atomic resolution from in situ

data collection at room temperature using

counter-diffusion-based low-cost microchips. (2020) Acta Crystallographica

Section D Structural Biology, 76 (8). 751-758. ISSN 2059-7983

DOI :

https://doi.org/10.1107/S2059798320008475

Open Archive Toulouse Archive Ouverte

OATAO is an open access repository that collects the work of Toulouse

researchers and makes it freely available over the web where possible

(2)



$WWDLQLQJDWRPLFUHVROXWLRQIURPin situGDWDFROOHFWLRQDWURRP

WHPSHUDWXUHXVLQJFRXQWHUGLIIXVLRQEDVHGORZFRVWPLFURFKLSV



-RVH$*DYLUD,VDDF5RGULJXH]5XL]6HUJLR0DUWLQH]5RGULJXH]6KLERP%DVX

6pEDVWLHQ7H\FKHQp$QGUHZ$0F&DUWK\DQG&KULVWRSK0XHOOHU'LHFNPDQ



(3)



)LJXUH6 6QDSVKRWSLFWXUHVRIWKHFRXQWHUGLIIXVLRQFU\VWDOOL]DWLRQH[SHULPHQWVRIJOXFRVH

LVRPHUDVHLPSOHPHQWHGLQ0\ODU 0WR0 DQG.DSWRQ .WR. PLFURFKLSV*UHHQSDWKRQWKH

FU\VWDOVVKRZVLQLWLDODQGILQDOSRLQWVRIWKHKHOLFDOWUDMHFWRULHVIROORZHGIRUGDWDFROOHFWLRQ

(4)

)LJXUH6 6QDSVKRWSLFWXUHVRIWKHFRXQWHUGLIIXVLRQFU\VWDOOL]DWLRQH[SHULPHQWVRIO\VR]\PH

LPSOHPHQWHGLQ0\ODU 0WR0 DQG.DSWRQ .WR. PLFURFKLSV*UHHQSDWKRQWKHFU\VWDOV

VKRZVLQLWLDODQGILQDOSRLQWVRIWKHKHOLFDOWUDMHFWRULHVIROORZHGIRUGDWDFROOHFWLRQ

(5)



)LJXUH6 6QDSVKRWSLFWXUHVRIWKHFRXQWHUGLIIXVLRQFU\VWDOOL]DWLRQH[SHULPHQWVRIWKDXPDWLQ

LPSOHPHQWHGLQ0\ODU 0WR0 DQG.DSWRQ .WR. PLFURFKLSV*UHHQSDWKRQWKHFU\VWDOV

VKRZVLQLWLDODQGILQDOSRLQWVRIWKHKHOLFDOWUDMHFWRULHVIROORZHGIRUGDWDFROOHFWLRQ





(6)



)LJXUH6 $6QDSVKRWSLFWXUHVRIWKH'R]RUVFRUHRXWSXWRIWKHGDWDFROOHFWLRQRIDWKDXPDWLQ

FU\VWDOH[HPSOLI\LQJWKHGLIILFXOWLHVLQSURSHUO\FHQWHULQJWKHFU\VWDOVLQWKHSODQHSHUSHQGLFXODUWRWKH

;UD\EHDP%+HOLFDOWUDMHFWRU\ WR IROORZHGGXULQJGDWDFROOHFWLRQ







(7)



)LJXUH6 6QDSVKRWRIWKH_)R)F_HOHFWURQGHQVLW\PDSVFRQWRXUHGDWVVKRZLQJWKHEXLOW

PRGHOVRIJOXFRVHLVRPHUDVH F\DQ O\VR]\PH JUHHQ DQGWKDXPDWLQ SLQN $&( EOXH DQG%'

( EURZQLVK PDSVFRUUHVSRQGWRFU\VWDOVJURZQLQ0\ODUDQG.DSWRQFKLSVUHVSHFWLYHO\



6XSSOHPHQWDU\PXOWLPHGLD

6XSSOHPHQWDU\0RYLH6UHSUHVHQWVDIXOOGDWDVHWFROOHFWHGE\URWDWLQJƒ





(8)

7DEOH6 6XPPDU\RIWKHGDWDFROOHFWLRQFRQGLWLRQVDQGILQDOVWDWLVWLFDOYDOXHVIRUJOXFRVHLVRPHUDVHFU\VWDOVJURZQLQWKH.DSWRQPLFURFKLSV

7KHILQDOVWDWLVWLFDOYDOXHVDUHIURP;6'$33;,$('1$RUDLPOHVV QRWHDVFFS 

'DWDVHW .*OX .*OX .*OX .*OX .*OX .*OX .*OX .*OX .*OX

'DWD$FTXLVLWLRQ         

(65)%HDPOLQH ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c           'LVWDQFH PP           1XPEHURILPDJHV          2VFLOODWLRQ R           7UDQVPLVVLRQ            ([SRVXUHWLPH PV           'DWD6WDWLVWLFV ;'6$33 ;,$ ;,$ ('1$;,$ DXWR352& ('1$FFS ('1$FFS ;,$ ;,$ 6SDFHJURXS , , , , , , , , , 8QLWFHOO DEF c           5HVROXWLRQ c   +LJKVKHOO                               8QLTXHUHIOHFWLRQV          5PHUJH                               ,ı , !                            &RPSOHWHQHVV                              0XOWLSOLFLW\                            &&                              %IDFWRU c           0RVDLFLW\ R          

(9)

'DWDVHW .*OXE .*OXE .*OXE .*OXE .*OX 'DWD$FTXLVLWLRQ

(65)%HDPOLQH ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c       'LVWDQFH PP       1XPEHURILPDJHV      2VFLOODWLRQ R       7UDQVPLVVLRQ        ([SRVXUHWLPH PV       'DWD6WDWLVWLFV ;,$ ;,$ ('1$FFS ('1$FFS ('1$FFS 6SDFHJURXS , , , , , 8QLWFHOO DEF c       5HVROXWLRQ c   +LJKVKHOO                 8QLTXHUHIOHFWLRQV      5PHUJH                   ,ı , !                &RPSOHWHQHVV                  0XOWLSOLFLW\                &&                  %IDFWRU c       0RVDLFLW\ R      

(10)

7DEOH6 6XPPDU\RIWKHGDWDFROOHFWLRQFRQGLWLRQVDQGILQDOVWDWLVWLFDOYDOXHVIRUJOXFRVHLVRPHUDVHFU\VWDOVJURZQLQWKH0\ODUPLFURFKLSV

7KHILQDOVWDWLVWLFDOYDOXHVDUHIURP;6'$33;,$('1$RUDLPOHVV QRWHDVFFS 

'DWDVHW 0\ODU*OX 0\ODU*OX 0\ODU*OX 0\ODU*OX 0\ODU*OX 0\ODU*OX 0\ODU*OX 0\ODU*OX 0\ODU*OX

'DWD$FTXLVLWLRQ         

(65)%HDPOLQH ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c           'LVWDQFH PP           1XPEHURILPDJHV          2VFLOODWLRQ R           7UDQVPLVVLRQ            ([SRVXUHWLPH PV          

'DWD6WDWLVWLFV ('1$FFS ('1$ SDUDOOHO3URF ;,$ ('1$ DXWR3URFFFS ('1$FFS ('1$FFS DXWR3URF

6SDFHJURXS , , , , , , , , , 8QLWFHOO DEF c           5HVROXWLRQ c   +LJKVKHOO                             8QLTXHUHIOHFWLRQV          5PHUJH                               ,ı , !                            &RPSOHWHQHVV                            0XOWLSOLFLW\                            &&                              %IDFWRU c           0RVDLFLW\ R          

(11)

'DWDVHW 0\ODU*OXE 0\ODU*OXE 0\ODU*OX 0\ODU*OX 0\ODU*OX

'DWD$FTXLVLWLRQ     

(65)%HDPOLQH ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c       'LVWDQFH PP       1XPEHURILPDJHV      2VFLOODWLRQ R       7UDQVPLVVLRQ        ([SRVXUHWLPH PV       'DWD6WDWLVWLFV DXWR352&FFS ('1$FFS ('1$FFS DXWR3URF ('1$FFS 6SDFHJURXS , , , , , 8QLWFHOO DEF c       5HVROXWLRQ c   +LJKVKHOO                8QLTXHUHIOHFWLRQV      5PHUJH                   ,ı , !                &RPSOHWHQHVV                  0XOWLSOLFLW\                &&                  %IDFWRU c       0RVDLFLW\ R      

(12)



7DEOH6 6XPPDU\RIWKHGDWDFROOHFWLRQFRQGLWLRQVDQGILQDOVWDWLVWLFDOYDOXHVIRUO\VR]\PHFU\VWDOVJURZQLQWKH0\ODUPLFURFKLSV

7KHILQDOVWDWLVWLFDOYDOXHVDUHIURP;6'$33;,$('1$RUDLPOHVV QRWHDVFFS 

'DWDVHW 0\ODU/]P 0\ODU/]P 0\ODU/]P 0\ODU/]P 0\ODU/]P 0\ODU/]P 0\ODU/]P 0\ODU/]P 0\ODU/]P

'DWD$FTXLVLWLRQ         

(65)%HDPOLQH ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c           'LVWDQFH PP           1XPEHURILPDJHV          2VFLOODWLRQ R           7UDQVPLVVLRQ            ([SRVXUHWLPH PV          

'DWD6WDWLVWLFV ;'6$33FFS DXWR3URFFFS ;'6$33FFS SDUDOOHO ;'6$33FFS ('1$FFS ;'6$33 ('1$ DXWR3URFFFS

6SDFHJURXS 3 3 3 3 3 3 3 3 3 8QLWFHOO D EF c           5HVROXWLRQ c   +LJKVKHOO                             8QLTXHUHIOHFWLRQV          5PHUJH                               ,ı , !                            &RPSOHWHQHVV                              0XOWLSOLFLW\                            &&                              %IDFWRU c           0RVDLFLW\ R          

(13)

'DWDVHW 0\ODU/]PE 0\ODU/]PE 0\ODU/]PE 0\ODU/]PE 0\ODU/]PF

'DWD$FTXLVLWLRQ     

(65)%HDPOLQH ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c       'LVWDQFH PP       1XPEHURILPDJHV      2VFLOODWLRQ R       7UDQVPLVVLRQ        ([SRVXUHWLPH PV       'DWD6WDWLVWLFV ;'6$33 ('1$ ('1$ DXWR3URFFFS DXWR3URFFFS 6SDFHJURXS 3 3 3 3 3 8QLWFHOO D EF c       5HVROXWLRQ c   +LJKVKHOO                 8QLTXHUHIOHFWLRQV      5PHUJH                   ,ı , !                &RPSOHWHQHVV                  0XOWLSOLFLW\                &&                  %IDFWRU c       0RVDLFLW\ R      

(14)



7DEOH6 6XPPDU\RIWKHGDWDFROOHFWLRQFRQGLWLRQVDQGILQDOVWDWLVWLFDOYDOXHVIRUO\VR]\PHFU\VWDOVJURZQLQWKH.DSWRQPLFURFKLSV

7KHILQDOVWDWLVWLFDOYDOXHVDUHIURP;6'$33;,$('1$RUDLPOHVV QRWHDVFFS  'DWDVHW ./]P ./]P ./]P ./]P ./]P ./]P ./]P ./]P ./]P 'DWD$FTXLVLWLRQ  (65)%HDPOLQH ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c           'LVWDQFH PP           1XPEHURILPDJHV          2VFLOODWLRQ R           7UDQVPLVVLRQ            ([SRVXUHWLPH PV           'DWD6WDWLVWLFV ('1$ ;,$ ('1$FFS ('1$FFS ('1$FFS DXWR3URFFFS SDUDOOHO ('1$FFS ('1$FFS 6SDFHJURXS 3 3 3 3 3 3 3 3 3 8QLWFHOO D EF c           5HVROXWLRQ c   +LJKVKHOO                           8QLTXHUHIOHFWLRQV          5PHUJH                               ,ı , !                            &RPSOHWHQHVV                              0XOWLSOLFLW\                            &&                              %IDFWRU c           0RVDLFLW\ R          

(15)

'DWDVHW ./]PE 'DWD$FTXLVLWLRQ  (65)%HDPOLQH ,'% 'HWHFWRUW\SH 3LODWXV0 :DYHOHQJWK c   'LVWDQFH PP   1XPEHURILPDJHV  2VFLOODWLRQ R   7UDQVPLVVLRQ    ([SRVXUHWLPH PV   'DWD6WDWLVWLFV SDUDOOHO 6SDFHJURXS 3 8QLWFHOO D EF c   5HVROXWLRQ c   +LJKVKHOO     8QLTXHUHIOHFWLRQV  5PHUJH       ,ı , !    &RPSOHWHQHVV      0XOWLSOLFLW\    &&      %IDFWRU c   0RVDLFLW\ R  

(16)



7DEOH6 6XPPDU\RIWKHGDWDFROOHFWLRQFRQGLWLRQVDQGILQDOVWDWLVWLFDOYDOXHVIRUWKDXPDWLQFU\VWDOVJURZQLQWKH.DSWRQPLFURFKLSV

7KHILQDOVWDWLVWLFDOYDOXHVDUHIURP;6'$33;,$('1$RUDLPOHVV QRWHDVFFS  'DWDVHW .7KDX .7KDX .7KDX .7KDX 'DWD$FTXLVLWLRQ     (65)%HDPOLQH ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c      'LVWDQFH PP      1XPEHURILPDJHV     2VFLOODWLRQ R      ([SRVXUHWLPH PV      7UDQVPLVVLRQ       'DWD6WDWLVWLFV DXWR3URFFS DXWR3URFFS ;,$ ('1$FFS 6SDFHJURXS 3 3 3 3 8QLWFHOO DEF c      5HVROXWLRQ c   +LJKVKHOO              8QLTXHUHIOHFWLRQV     5PHUJH                ,ı , !             &RPSOHWHQHVV               0XOWLSOLFLW\             &&               %IDFWRU c      0RVDLFLW\ R     

(17)

7DEOH6 6XPPDU\RIWKHGDWDFROOHFWLRQFRQGLWLRQVDQGILQDOVWDWLVWLFDOYDOXHVIRUO\VR]\PHFU\VWDOVJURZQLQWKH0\ODUPLFURFKLSV

7KHILQDOVWDWLVWLFDOYDOXHVDUHIURP;6'$33;,$('1$RUDLPOHVV QRWHDVFFS 

'DWDVHW 0\ODU7KDX 0\ODU7KDX 0\ODU7KDX 0\ODU7KDXE 0\ODU7KDX 0\ODU7KDX 0\ODU7KDX 0\ODU7KDX 0\ODU7KDX

'DWD$FTXLVLWLRQ

(65)%HDPOLQH ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c           'LVWDQFH PP           1XPEHURILPDJHV          2VFLOODWLRQ R           7UDQVPLVVLRQ            ([SRVXUHWLPH PV           'DWD6WDWLVWLFV DXWR3URFFS ;'6$33FFS ('1$FFS ;'6$33FFS ;,$FFS ('1$FFS ;,$ ('1$FFS ('1$FFS 6SDFHJURXS 3 3 3 3 3 3 3 3 3 8QLWFHOO DEF c           5HVROXWLRQ c   +LJKVKHOO                             8QLTXHUHIOHFWLRQV          5PHUJH                               ,ı , !                            &RPSOHWHQHVV                              0XOWLSOLFLW\                            &&                              %IDFWRU c           0RVDLFLW\ R          

(18)

'DWDVHW 0\ODU7KDXE 0\ODU7KDXE 0\ODU7KDX 0\ODU7KDX 0\ODU7KDXE 'DWD$FTXLVLWLRQ

(65)%HDPOLQH ,'% ,'% ,'% ,'% ,'%

'HWHFWRUW\SH 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0 3LODWXV0

:DYHOHQJWK c       'LVWDQFH PP       1XPEHURILPDJHV      2VFLOODWLRQ R       7UDQVPLVVLRQ        ([SRVXUHWLPH PV       'DWD6WDWLVWLFV ;,$ ;,$ ;,$FFS ;,$FFS ;,$FFS 6SDFHJURXS 3 3 3 3 3 8QLWFHOO DEF c       5HVROXWLRQ c   +LJKVKHOO                 8QLTXHUHIOHFWLRQV      5PHUJH                   ,ı , !                &RPSOHWHQHVV                  0XOWLSOLFLW\                &&                  %IDFWRU c       0RVDLFLW\ R      

(19)

7DEOH6 5HVROXWLRQOLPLWRIWKHWKUHHPRGHOSURWHLQVO\VR]\PHWKDXPDWLQDQGJOXFRVHLVRPHUDVHREWDLQHGIURPPLFURIOXLGLFV\VWHPVFRPSDUHGZLWKWKH

GDWDREWDLQHGLQWKLVZRUN

0DWHULDO 5HVROXWLRQOLPLW c  /\VR]\PH 7KDXPDWLQ *OXFRVH,VRPHUDVH 3XEOLFDWLRQ .DSWRQ    7KLVZRUN 0\ODU    7KLVZRUN &2&   1J et al.    'KRXLE et al.    3LQNHU et al.  3'06  +DQVHQ et al.   'KRXLE et al.  300$  'KRXLE et al.  'URSOHW V\VWHP  *HUGWV et al.   +H\PDQQ et al.  .DSWRQ    *LFTXHO et al.  3RO\FDUERQDWH 

Références

Documents relatifs