Role of transcription factor KLF11 and its diabetes-associated gene variants in pancreatic beta cell function

They also inhibited suppression of STZ-induced insuli- noma cell death by DPQ (Fig. 4b, bars 6–9 from the left for okadaic acid and vanadate), supporting that inhibition of

Since both hormones failed to activate MAP kinase in these cells, it is strongly suggested that activation of the JAK-STAT pathway is the major signalling event

Nuclear extracts from both HNF-1α # 15 and DNHNF- 1α # 31 cells cultured in the absence of doxycycline con- tained similar amounts of HNF-1α binding activity (Figure 2A),

In the pancreatic /3-celi, mitochondrial metaboiism plays a pivotai role in the generation of signais coupling glucose recognition to insulin secretion [11,27,30,35].. Metabolism

Our results show that the defec- tive glucose-induced insulin secretion observed after H 2 O 2 treatment correlates with altered mitochondrial activation seen as a loss of

F IG. Induction of WT-HNF1␣ and DN-HNF1␣ regulates the HNF1␣ mRNA expression and HNF1␣ promoter luciferase activity through altered HNF4 ␣ binding. A, for EMSA, the

The percentage of labelled proinsulin directed to the regulated secretory pathway was calculated as 100 − x, where x is the percentage of labelled proinsulin released without

To study the role of calpain on ␤ -cell function, ␤ -cells were plated on PLL or 804G matrix and preincubated for 24 h with calpeptin, and short-term insulin secretion was then