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OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible.
This is an author-deposited version published in : http://oatao.univ-toulouse.fr/
Eprints ID : 11952
To cite this version : Evon, Philippe and Colas, Dorothée and
Pontalier, Pierre-Yves and Rigal, Luc The twin-screw extrusion
technology, an original solution for the extraction of proteins from sunflower and alfalfa. (2010) In: 101st AOCS Annual Meeting &
Expo, 16 May 2010 - 19 May 2010 (Phoenix, Arizona, United States)
Any correspondance concerning this service should be sent to the repository
The twin-screw extrusion technology, an original solution
for the extraction of proteins from sunflower and alfalfa
Ph. Evon*, 1, 2, D. Colas1, 2, P.Y. Pontalier1, 2, L. Rigal1, 2
1
Université de Toulouse; INP; LCA (Laboratoire de Chimie Agro-industrielle); ENSIACET, 4 Allée Emile Monso, 31432 Toulouse Cedex 4, France
2
INRA; LCA (Laboratoire de Chimie Agro-industrielle); 31432 Toulouse Cedex 4, France
Keywords: Twin-screw extrusion, Protein extraction, Sunflower, Alfalfa.
Twin-screw extrusion has been used for the protein extraction from sunflower and alfalfa. Thermo-mechanical fractionation and aqueous extraction are conducted simultaneously to collect separately a liquid extract and a solid raffinate.
From sunflower whole plant, squeezing in the reversed screws is favored by the fibers abundance in the stalk, and it enables L/S separation. Protein yield is 44%, in the best conditions, and lipids are partly co-extracted. Water-soluble proteins are in an aqueous extract and in two O/W emulsions due to their surface-active properties. Hence, the oil is co-extracted in the form of emulsions stabilized by proteins at interface.
Proteins can be collected in the aqueous extract by isoelectric precipitation. Emulsions are usable for oil production. Their demulsification with ethanol produces a precipitate rich in proteins with low denaturation level.
From alfalfa whole plant, the highest protein yield in the aqueous extract (called green juice) is 25% when water is added in the extruder. Water-soluble and water-insoluble green juice proteins can be fractionated thanks to L/L extraction in sunflower oil and ammonium sulfate precipitation.
Some other molecules are co-extracted (polysaccharides, phenolic compounds). They can be purified with ultrafiltration or chromatography.
__________
* Corresponding author. Tel.: + 33 (0)5 62 44 60 80; fax: + 33 (0)5 62 44 60 82.
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