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Clostridium perfringens urease genes are plasmid-borne.

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Figure

FIG. 1. Physical map of C. perfringens urease subunits gene cluster. The boxes, labeled ureA, ureB, and ureC, indicate the physical positions of each of the ure open reading frames
FIG. 2. Amino acid sequence similarities between C. perfringens urease sub- sub-units and those from L
FIG. 4. Immunodetection of urease subunits. Proteins in crude cell extracts of C. perfringens CP76 and CPN50 grown in minimal medium were separated by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose filters, and then detected with ant
FIG. 5. Localization of urease genes on plasmids. Pulsed-field gel electro- electro-phoresis and hybridization analysis of intact genomic DNA which was either undigested, digested with I-CeuI, or digested with ApaI stained with ethidium bromide (A), hybrid
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