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Role of Cys221 and Asn116 in the zinc-binding sites of the Aeromonas hydrophila metallo-beta-lactamase.

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Academic year: 2021

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Table 1: List of mutagenic primers used to generate mutants,  Primers  Sequences  N116Sfor  5'-GAGGTGATCAACACCAGCTACCACACCGACC-3'  N116Srev  5'-GGTCGGTGTGGTAGCTGGTGTTGATCACCTC-3'  NU6Cfor  5'-GAGGTGATCAACACCTGCTACCACACCGACC-3'  N116Crev  5'-GGTCGGTGTGGTAGC
Table 2: Kinetic parameters of the wild-type enzyme (WT) and the mutants N116S, Nl 16C and N116H
Table 4: kcat and kcat/Km values for the wild type protein (WT) and the Nl 16S, N116C and N116H mutants  (from Table 2) expressed as the ratio of the parameter measured against imipenem vs
Figure 1 :  Residual activities measured in the presence of increasing concentrations of zinc

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