Table S1. Strains and plasmids.
Strains Characteristics Reference or source
DH5 K-12, F- 80 Δ (lacZYA-argF) endA1
recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1
Bethesda Research Laboratories
7122 APEC O78:K80:H9, gyrA Nalr
7273 7122 tsh::tetAR(B), Nalr Tcr
7274 7273 cured of pAPEC-1, Nalr
Plasmids
pBluescript SKII AmpR cloning vector Stratagene
pYA3108 tsh gene encoded on a 7 kb ClaI
References
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Table S2. Primers, amplicons and positive controls for the virulence genes used to characterize APEC SEPT362 by PCR.
Activity Primer Sequence (5’ 3’) Amplicon size
(bp) Positive control Reference Toxin (LT) LT-F ACGGCGTTACTATCCTCTC 273 PCAW LT-R TGGTCTCGGTCAGATATGTG Toxin (STp) STp-F TCTTTCCCCTCTTTTAGTCAG 166 PCAY STp-R ACAGGCAGGATTACAACAAAG Toxin (STh) STh-F TTCACCTTTCCCTCAGGATG 120 PCAY STh-R CTATTCATGCTTTCAGGACCA Toxin (Stx1) stx1-F ATAAATCGCCATTCGTTGACTAC 180 EDL933 stx2-R AGAACGCCCACTGAGATCATC Toxin (Stx2) stx2-F GGCACTGTCTGAAACTGCTCC 255 EDL933 stx2-R TCGCCAGTTATCTGACATTCTG Toxin (CNF-1) cfn1-F CATTCAGAGTCCTGCCCTCATTATT 498 FVL16 cnf1-R AAGATGGAGTTTCCTATGCAGGAG Toxin (CNF-2) cfn2-F TATCATACGGCAGGAGGAAGCACC 1,241 EC895 cfn2-F GTCACAATAGACAATAATTTTCCG Toxin (CDT-I) cdt-Is-F CAATAGTCGCCCACAGGA 411 EC457 cdt-IIas-R ATAATCAAGAACACCACCAC Toxin (EAST-1) EAST1-F TGCCATCAACACAGTATATCC 116 APECO1 EAST1-R TCAGGTCGCGAGTGACGGC
(Tsh) tsh-R CTTCCGATGTTCTGAACGT SPATE (Sat) sat-F TGCTGGCTCTGGAGGAAC 667 FVL35 sat-R TTGAACATTCAGAGTACCGGG SPATE (Pic) pic-F ACTGGATCTTAAGGCTCAGG 409 FVL16 pic-R TGGAATATCAGGGTGCCACT SPATE (Vat) vat-F TCCTGGGACATAATGGTCAG 981 APECO1 vat-R GTGTCAGAACGGAATTGT SPATE (SigA) sigA-F CCGACTTCTCACTTTCTCCCG 430 CF2 sigA-R CCATCCAGCTGCATAGTGTTTG SPATE (Pet) pet-F GGCACAGAATAAAGGGGTGTTT 302 042 pet-R CCTCTTGTTTCCACGACATAC SPATE (SepA) sepA-F GCAGTGGAAATATGATGCGGC 794 CF2 sepA-R TTGTTCAGATCGGAGAAGAACG SPATE (EatA) eatA-F CAGGAGTGGGAACATTAAGTCA 743 ETEC23 eatA-R CGTACGCCTTTGATTTCAGGAT SPATE (EspP) espP-F GTCCATGCAGGGACATGCCA 547 EDL933 espP-R TCACATCAGCACCGTTCTCTAT SPATE (EspC) espC-F TAGTGCAGTGCAGAAAGCAGTT 301 E2348/69 espC-R AGTTTTCCTGTTGCTGTATGCC Adhesin (ECP) G268** GTGACATGGCAAAATGATTACAGC 1974 EDL933 G273*** TCACGGGAATGAACTTATCACCC Adhesin (Type 1
fimH-F TGCAGAACGGATAAGCCGTGG 508 U-8301/12
fimbria) Adhesin (Long polar fimbria A1-1) lpfAO127-F AGTTGGTGATAAATCACCAT 222 E2348/69 lpfAO127-R GTGCTGGATTCACCACTATTCATCG Adhesin (Long polar fimbria A1-2) lpfAO26-F AAGTCTGTATTTACTGCTATG 273 48C-A lpfAO26-R GAAATACAGAACGGTCTGA Adhesin (Long polar fimbria A1-3) lpfAO157/OI-141-F CTGCGCATTGCCGTAAC 412 EDL933 lpfAO157/OI-141-R ATTTACAGGCGAGATCGTG Adhesin (Long polar
lpfAONT:H10-F AAGTCTGTGTTTACCACTACT 273 SCI07
fimbria A1-4) Adhesin (Long polar fimbria A1-5)
lpfA ONT:H26-F GGTTGGTGACAAATCCCCG
250 O119
lpfA ONT:H26-R GAGAACCGTCTGGCCTGTTT
Adhesin (Long polar fimbria A2-1) lpfAO113-F GGTAGTCTGGCGTCGCCACAGA 207 O8 lpfAO113-R AATACGAATACCAACGCCG Adhesin (Long polar fimbria A2-2) lpfAO157/OI-154-F GCAGGTCACCTACAGGCGGC 525 EDL933 lpfAO157/OI-154-R CTGCGAGTCGGAGTTAGCTG Adhesin (Long lpfAO44-F GGTAGTCTGGCGTCACCACAGC 207 042 lpfAO44-R AATACGAATACCGACACCC
polar fimbria Adhesin (Curli) csgA-F ACTCTGACTTGACTATTACC 200 U-8301/12 csgA-R AGATGCAGTCTGGTCAAC Adhesin (S fimbrial adhesion) sfa/focDE-F CTCCGGAGAACTGGGTGCATCTTAC 410 FVL16 sfa/focDE-R CGGAGGAGTAATTACAACCTGGCA Adhesin (P fimbria) papC-F GTGGCAGTATGAGTAATGACCGTTA 200 FVL16 papC-R ATATCCTTTCTGAGGGATGCAATA Adhesin (afimbrial adhesin) afa/drab-F TAAGGAAGTGAAGGAGCGTG 809 FVL35 afa/drab-R CCAGTAACTGTCCGTGACA Adhesin (REPEC adherence locus) ral-F ATGGATAAGCGTACGCC 2,282 REPEC83/39 Adhesin (REPEC adherence ral-R AATACTAACTGGTAGGG
locus)
* Serine protease autotransporter ** located in the ecpR gene *** located in the ecpB gene
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