GABA, glutamine (GLN), and aspartate (ASP) release, content and total amino acid amounts [pmol]
6.11 Islet cell isolation
Islet cells were isolated by FACS analysis [36]. For this purpose, isolated islets were washed twice in PBS w/o Ca2+ and Mg2+. Washed islets were resuspended in 5ml dispersion buffer composed of 2ml 0.05% trypsin/EDTA, 5ml PBS w/o Ca2+ and Mg2+ and 5.25µl DNaseI (10mg/ml) and transferred into a non-adherent 6cm petri dish. Islets were dispersed mechanically by pipetting. The dispersed cells were filtered through a nylon cell
resuspended in ~1ml KRBH buffer and transferred into a pre-rinsed FACS tube. Cells were dissociated into beta and non-beta cells (α-cells) by autofluorescence-activated cell sorting using FACStar+. An argon laser illuminated the cells at 488nm, so that the emission at 510-550nm could be taken as a parameter for their flavin adenine dinucleotide (FAD) content. At 2.5mM glucose (in KRBH) single beta-cells display a 3-fold higher FAD fluorescence than non-beta cells. Selection of appropriate windows allowed the simultaneous isolation of single beta- and non-beta cells.
Sorted cells were centrifuged at 1600rpm for 10min. Pelleted cells were resuspended in RPMI-1640 medium containing 2.5mM glucose and seeded onto polyornithine-coated 24-well plates (10’000cells/24-well). Cells were cultured at 37°C overnight before performing a hormone assay. Details of the islet cell hormone assay are described in the submitted article.
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